19. Which cloning vector has the largest cloning capacities?
(A) Plasmid
(B) YAC
(C) BAC
(D) Cosmids

(E) Lambda vector

1. Which of the following statement is NOT correct for the regulation of lactose (lac) operon?(A) lac operon is repressible catabolic operon.(B) lac I repressor binds to operator of lac operon in the presence of glucose.(C) Catabolite gene activator protein (CAP) is a positive control of lac operon without cAMP.(D) 1,6-allolactose functions as a true inducer.(E) I-Z+/i+Z- diploid strain with lacZ inducibility in response to IPTG.

2. Which of the following statement is correct for the development of prophage?(A) Lambda repressor is expressed in the lysogenic bacteria cells.(B) Lambda repressor binds to OR1 to express Cro protein.(C) Lambda repressor binds to Or2 to inhibit the expression of itself.(D) Cro binds to OR1 to inhibit the expression of lambda repressor.(E) Lysogeny is terminated by proteolysis of N protein.

3. Which of the following factor is NOT involved in the efficiency of eukaryotic transcription?(A) Histone modification(B) DNAmethylation on deoxycytidine of CpG island(C) Enhancer and silencer(D) microRNA (miRNA)(E) Deletion of genomic information in B cells

4. Which ofthe following statement is NOT correct for the characteristics of enhancers? (A) Work when located long distances from the promoter. (B) Work when upstream or downstream from the promoter. (C) Work when only one direction is same as TATA box. (D) Work through heterologous promoters. (E) Work by binding one or more proteins.

5. Which ofthe following method is NOT for mRNA quantitation?(A) Southern blot analysis(B) Reverse transcription-quantitative polymerase chain reaction(C) cDNA microarray(D) Transcriptome analysis(E) Next generation sequencing

6.Which of the following statement is NOT correct for the evaluation of SARS-CoV-2 infection?(A) Lower Ct (threshold cycle) value represents the higher titer of the virus.(B) Genomic RNA should be converted to cDNA before polymerase chain reaction for the quantitation of virus titers.(C) Identification of SARS-CoV-2 variant and sequences is critical to judge the cross-contamination.(D) Amicron variant contains more mutations compared to delta variant.(E) mRNA of the nucleocapsid protein is currently used for the vaccination.

7. Which of the following statement is NOT correct for the regulation of trp operon?(A) trp operon is repressible anabolic operon.(B)In the presence of high [Trp], the attenuated mRNA of trp operon is expressed.(C)In the presence of low [Trp], trp mRNA is synthesized.(D) The formation of mRNA stem-loop 3 and 4 causes rho-dependent termination.(E) Low level of [Trp-tRNAPtrp] causes the formation of mRNA stem-loop 2 and 3.

8.Which of the following transcription regulatory protein contains zinc finger binding motif?(A) lac repressor(B) Homeo box protein(C) Transcription factor TFIIIA(D) Fos and Jun(E) c-myc

9. Which enzyme is involved in the ER oligosaccharides processing?(A) Oligosaccharide:protein transferase(B) N-acetyl glucosamintransferase(C) Galactose transferase(D) Fucosyltransferase(E) Mannosidase

10. Which description about proteogłycan glycosylation is correct?(A) The posttranslational modification for directing glycoprotein transfer from ER to lysosome(B) N-acetylglucosamin Asn linkage(C) -Ser-X-Gly-Gly is the attachment site(D) N-acetylgalactosamin Asn linkage(E) Containing Gal-Gal-Xyl trisaccharides core

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